Biotechnology Restriction Enzyme Analysis

Configure & Price

Reference: IS.3709

Each kit is meant for

Quantity

$138.00

In this lab students will understand the action and specificity of restriction enzymes, while learning the components involved in the process of DNA electrophoresis and the role of each component as it applies to the entire process of electrophoresis. Students will perform the electrophoresis process on DNA samples treated with different restriction enzymes and construct a standard curve using a known DNA sample while determining the approximate size of the DNA fragments in unknown samples.

Includes:

200mL 0.8% prepared agarose
500mL 5X TBE buffer
60mL 20X DNA stain
100ul DNA marker (Lambda DNA HindIII digest)
100ul Sample #1 (Lambda DNA)
100ul Sample #2 (Lambda DNA BstEII digest)
100ul Sample #3 (Lambda DNA EcoRI digest)
100ul Sample #4 (Lambda DNA BstEII digest)

Reference IS.3709

Items needed, but not included: Agarose electrophoresis chamber
Electrophoresis power supply
Micropipettes w/ 10μl micropipette tips
Gel staining trays (container for flat agarose gels)
Microwave or hot water bath
Distilled or deionized water
Quantity: 8 lab groups
Includes: Teachers manual and Student Study Guide copymasters

Resources

Safety Data Sheet - (IS3705)

Investigation-5-SDS

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